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By “artificial compound” is supposed herein any molecule and that is neither By natural means produced by the host issue (or by a subject matter of a similar species as being the host species) nor by another organism, specifically an organism from A different species, from An additional gender, from One more family members, from A further course or from One more kingdom.
In a particular embodiment, the CRISPR enzyme is any Cas protein, specifically any Cas9 protein, As an example any Obviously occurring bacterial Cas9 in addition to any variants, chimeras, homologs or orthologs thereof.
In a selected embodiment, the conditional origin of replication is surely an origin of replication, the replication of which depends upon the presence of a number of supplied protein, peptid, RNA, nucleic acid, molecule or any mixture thereof.
By “modulating the microbiome perform” is supposed herein changing the operate of unique species or strains of claimed microbiome, for instance by producing particular species or strains Categorical specific molecules, or by 點此進一步了解 producing distinct species or strains prevent expressing unique molecules.
comprising reported nucleic acid of fascination, whereby explained vector further comprises a conditional origin of replication that's inactive during the qualified receiver bacterial cell but is active in the donor bacterial mobile, and said vector is devoid of antibiotic resistance marker,
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in the event the antibiotic resistance gene is situated in the bacterium over a plasmid with out habit devices, it is possible to reduce the antibiotic resistance by cleavage possibly in the antibiotic resistance gene or anyplace else inside the plasmid.
In some embodiments with the current creation, a promoter might or might not be utilised together with an “enhancer,” which refers to your ds-acting regulatory sequence involved in the transcriptional activation of a nucleic acid sequence downstream of the promoter. The enhancer may be Positioned at any practical location ahead of or following the promoter.
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wherein said vector is usually a phagemid or recombinant phage nucleic acid vector, claimed vector comprising a conditional origin of replication which happens to be inactive in the specific receiver bacterial cell but is Lively within a donor bacterial mobile, wherein said conditional origin of replication is SEQ ID NO: four which is the origin of replication with the phage-inducible chromosomal island (PICI) on the Escherichia coli strain CFT073, or mentioned origin of replication is modified being the sequence of SEQ ID NO: six or SEQ ID NO: seven, and wherein mentioned conditional origin of replication is active in explained donor bacterial cell mainly because reported donor bacterial cell expresses a primase-helicase comprising SEQ ID NO: 8 and whereby claimed vector is devoid of antibiotic resistance marker.
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By “receiver bacterial mobile’ is meant herein any bacterium from your host microbiome which can be particularly qualified to become sent with explained nucleic acid of desire.
within a desired embodiment, the concentrate on microbes belong on the Firmicutes phylum. inside of a preferred embodiment, the focus on gene while in the focus on micro organism is part from the transketolase N superfamily.
In a specific embodiment, claimed conditional origin of replication is Lively in reported donor bacterial cell for the reason that stated donor bacterial mobile expresses reported supplied protein, peptid, RNA, nucleic acid, molecule or any mixture thereof.
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